The Laboratory services at Dr. Makkis Diabetes Specialities Center is unique in using advanced technology and having fully automated walk away systems in the department of Biochemistry, Hematology, Pathology, Microbiology and Serology which provide round the clock services, seven days a week. The laboratory is equipped with state-of-the-art equipment and has a team of efficient, motivated, knowledgeable and qualified biochemists and technical staff who are constantly involved in producing accurate and reliable results and upholding quality assurance.



All the blood samples in the laboratory testing are collected using vacutainer system of collection and labeled with bi-directional barcodes which avoids cross contamination and pre analytical error. Bi-directional barcoded samples are directly fed into the respective analyzer and results are automatically transmitted to Laboratory Information System (LIS) without manual entry to avoid analytical and post analytical error.

The laboratory has been accredited by the following National and International organizations.



The laboratory’s Quality Monitoring system helps to improve the accuracy and reliability of results in term of internal Quality Control (QC) as well as external proficiency testing materials. Internal QC material is analyzed at predetermined intervals along with patient samples to monitor systemic and random errors. These materials are traceable to international certified reference material so that accuracy of measurements is monitored periodically.



Department of Hematology
The Hematology Department provides laboratory services for the diagnosis and treatment of all blood related disorders such as anemia, bleeding disorders and malignant conditions related to blood cells such as leukemia, lymphoma and myeloma. Complete Blood Counts (CBC) are analysed by Sysmex XT1800i (80 samples/hour) analyzer and Coagulation tests are performed in the automated Random Access Coagulation analyzer Sysmex CA 50.

Department of Clinical Pathology
Urine analysis is used in detecting a variety of renal, pre-renal, urinary tract diseases. A semi-automated Cobas u 411 (600 test strips/hour) is used to rapidly detect PH, specific gravity, protein, glucose, bilirubin and other compounds in urine. The technician examines urine for microscopic elements such as red blood cells, pus cells, epithelial cells, casts and crystals.

SNO ANALYTES METHODS
1. Glucose Hexokinase / Kinetic
2. Urea Urease GLDH / UV Kinetic
3. Creatinine Jaffe / Kinetic
4. Cholesterol Total CHOD-POD / End Point
5. Triglycerides GPO-PAP / End Point
6. HDL Cholesterol Immunoinhibition
7. AST IFCC without P5P / UV Kinetic
8. ALT IFCC without P5P / UV Kinetic
9. Alkaline Phosphatase DEA Buffer
10. Total Protein Biuret Method / End Point
11. Albumin BCG Method / End Point
12. Bilirubin Total DPD/ End Point
13. Bilirubin Direct DPD / End Point
14. Gamma GT Szasz IFCC / Kinetic
15. Amylase G7 PNP / Kinetic
16. Sodium Ion Selective Electrode
17. Potassium Ion Selective Electrode
18. Chloride Ion Selective Electrode
19. Bicarbonate PEP / End Point
20. CPK NAC activated
21. Calcium Arsanazo / End Point
22. Phosphorus Molybdate UV
23. Uric Acid Uricase / End Point
24. Lactate Lactate to Pyruvate
25. C – Peptide ECLIA
26. Insulin ECLIA
27. Apo A Immunoturbidimetry
28. Apo B Immunoturbidimetry
29. Beta Hydroxybutrate Enzymatic / BHB dehydrogenase
30. Fructosamine NBT/Kinetic
31. HbA1c HPLC
32. FT3 ECLIA
33. FT4 ECLIA
34. TSH ECLIA
35. Total PSA ECLIA
36. Free PSA ECLIA
37. Lipase Enzymatic with colipase
38. IA2 Antibody ELISA
39. GAD Antibody ELISA
40. Osmolality (Serum & Urine) Calculated
41. Microalbumin (Urine) Immunoturbidimetry
42. Protein (Urine) Pyrogallol Red
43. Sodium (Urine) Ion Selective Electrode
44. Potassium (Urine) Ion Selective Electrode
45. Chloride (Urine) Ion Selective Electrode
46. hs CRP Immunoturbidimetry
47. Creatinine (Urine) Jaffe / Kinetic
48. Glucose (Urine) Hexokinase / Kinetic
49. Urea (Urine) Urease GLDH / UV Kinetic
50. Folate ECLIA
51. Ferritin ECLIA
52. Vitamin B12 ECLIA
53. Iron TPTZ-no deproteinization
54. UIBC Nitroso-PSAP
55. TIBC Calculation
56. 25 Hydroxyvitamin D ECLIA
57. Testosterone ECLIA
58. Cortisol ECLIA

Our laboratory’s quality is assured by the International and National EXTERNAL QUALITY ASSURANCE (EQA) programme conducted by

Department of Clinical Biochemistry

COLLEGE OF AMERICAN PATHOLOGISTS (CAP), USA (The second such lab in Chennai)

NATIONAL ACCREDITATION BOARD FOR TESTING AND CALIBRATION LABORATORIES (NABL UNDER ISO/IEC 15189), Pakistan.

World’s First ISO 9001:2008 Certified Diabetes laboratory,
College of American Pathologists (CAP), Illinois, USA
Bio-Rad Laboratories, California, USA
Christian Medical College (CMC), Vellore, India
All India Institute of Medical Sciences (AIIMS), New Delhi, India
Amala Institute of Medical Sciences (AIMS), Thrissur, India

The initial step towards a cure is the diagnosis. The faster and more accurate the diagnosis, the easier and better the cure. The biochemistry department goes the extra mile by being committed to ensuring accuracy and precision in specimen handling, clinical testing and making available the latest investigation in the field, so as to facilitate faster diagnosis.
The Biochemistry Department performs more than 3000 tests per day pertaining to 48 parameters. All the biochemistry assays are performed in high throughput analyzers.

Beckman Coulter AU2700 & AU480 chemistry analyzer (throughput 2100 tests/hr)
Roche Cobas e601 Immunoassay module for selective sample processing of immunological parameters with the ECL technology. (throughput 170 tests/hr)
India’s first Bio-Rad Variant II turbo Glycated Hemoglobin (HbA1c) analyzer (throughput 40 test/hr), which has been certified by the National Glycohaemoglobin
Standardization Program (NGSP), USA.

SEBIA CAPILLARYS 2 Flex Piercing System, a fully automated solution for high quality HbA1c testing (throughput 36 test/hr)
EasyLyte Na/K & Cl analyzers, a completely automated, microprocessor controlled electrolyte system which used to analyse sodium, potassium, and chloride in whole blood, serum, plasma, or urine.

Bio-Rad iMark™ microplate ELISA reader for the analysis of Anti-GAD, Anti-IA2, Tissue transglutaminase antibody (TTG), Zinc Transporter 8 antibody etc

Department of Microbiology and Serology
The Microbiology and Serology Department has high-end facilities for processing and reporting patient’s specimens in the field of diagnostic bacteriology, virology and parasites, which may cause a range of diseases.

In addition to identification, the department is actively involved in infection control activities of the hospital.

60 Minutes Reports
The samples are processed immediately; the reports are validated continuously and released. The turnaround time after postprandial sample reaches the lab, is on an average 60 minutes. The results are available within this period which reduces waiting time of patients.

Home Blood Collection
The Laboratory undertakes house collection with prior appointment within Chennai. For details contact Front Office. (91-44) 43968888/28359048/ 28359050/ 28353038

SNO ANALYTES METHODS
II. CLINICAL PATHOLOGY
59. Urine Sugar Reagent Strip method
60. Urine Protein Reagent Strip method
61. Urine Acetone Reagent Strip method
62. Urine pH Reagent Strip method
63. Urine Specific gravity Reagent Strip method
64. Urine Colour Reagent Strip method
65. Urine Appearance Reagent Strip method
66. Urine Bile salts Reagent Strip method
67. Urine Bile pigments Reagent Strip method
68. Urine Urobilinogen Reagent Strip method
69. Urine Deposits Microscopy
70. Urine Macroscopic and Microscopic Examination Microscopy
71. Motion Occult Blood GUAIAC Method
III. HAEMATOLOGY & IMMUNOHAEMATOLOGY
72. Complete blood cell count Flow cytometry method using semi conductor laser
73. Erythrocyte Sedimentation Rate Seditainer Method
74. Smear for MF Microscopy by Thin Smear
75. Smear for MP Microscopy / Thick and Thin Smear
76. Peripheral Smear Study Microscopy / Leishman’s Stain
77. PT Photo Optical Detection Method
78. aPTT Photo Optical Detection Method
79. Blood Grouping & Rh Typing Tube Method
80. Optimal Malaria Rapid Method
81. Bleeding Time Ivy’s Method
82. Clotting Time Lee and White Method
IV. MICROBIOLOGY & SEROLOGY
83. Widal Test Tube agglutination
84. RA Factor Latex Agglutination
85. HBsAg Rapid Method
86. Anti-HCV Rapid Method
87. RPR Flocculation
88. HIV Rapid Method Tri-Dot
89. Gram Stain Direct Microscopy
90. CULTURES – Urine, Tissue, Pus, Sputum, Nasal swab, Throat swab, Stool and Body fluids including synovial fluid, aspirate fluid, pleural fluid, ascitic fluid and liver abscess fluid Culture –Aerobic
91. Sensitivity – all clinical specimens Disc Diffusion by Kirby Bauer Method
92. AFB stain – Sputum Ziehl Neelsen stain / Direct Microscopy