The Laboratory services at Dr. Makkis Diabetes Specialities Center is unique in using advanced technology and having fully automated walk away systems in the department of Biochemistry, Hematology, Pathology, Microbiology and Serology which provide round the clock services, seven days a week. The laboratory is equipped with state-of-the-art equipment and has a team of efficient, motivated, knowledgeable and qualified biochemists and technical staff who are constantly involved in producing accurate and reliable results and upholding quality assurance.
All the blood samples in the laboratory testing are collected using vacutainer system of collection and labeled with bi-directional barcodes which avoids cross contamination and pre analytical error. Bi-directional barcoded samples are directly fed into the respective analyzer and results are automatically transmitted to Laboratory Information System (LIS) without manual entry to avoid analytical and post analytical error.
The laboratory has been accredited by the following National and International organizations.
The laboratory’s Quality Monitoring system helps to improve the accuracy and reliability of results in term of internal Quality Control (QC) as well as external proficiency testing materials. Internal QC material is analyzed at predetermined intervals along with patient samples to monitor systemic and random errors. These materials are traceable to international certified reference material so that accuracy of measurements is monitored periodically.
Department of Hematology
The Hematology Department provides laboratory services for the diagnosis and treatment of all blood related disorders such as anemia, bleeding disorders and malignant conditions related to blood cells such as leukemia, lymphoma and myeloma. Complete Blood Counts (CBC) are analysed by Sysmex XT1800i (80 samples/hour) analyzer and Coagulation tests are performed in the automated Random Access Coagulation analyzer Sysmex CA 50.
Department of Clinical Pathology
Urine analysis is used in detecting a variety of renal, pre-renal, urinary tract diseases. A semi-automated Cobas u 411 (600 test strips/hour) is used to rapidly detect PH, specific gravity, protein, glucose, bilirubin and other compounds in urine. The technician examines urine for microscopic elements such as red blood cells, pus cells, epithelial cells, casts and crystals.
SNO
ANALYTES
METHODS
1.
Glucose
Hexokinase / Kinetic
2.
Urea
Urease GLDH / UV Kinetic
3.
Creatinine
Jaffe / Kinetic
4.
Cholesterol Total
CHOD-POD / End Point
5.
Triglycerides
GPO-PAP / End Point
6.
HDL Cholesterol
Immunoinhibition
7.
AST
IFCC without P5P / UV Kinetic
8.
ALT
IFCC without P5P / UV Kinetic
9.
Alkaline Phosphatase
DEA Buffer
10.
Total Protein
Biuret Method / End Point
11.
Albumin
BCG Method / End Point
12.
Bilirubin Total
DPD/ End Point
13.
Bilirubin Direct
DPD / End Point
14.
Gamma GT
Szasz IFCC / Kinetic
15.
Amylase
G7 PNP / Kinetic
16.
Sodium
Ion Selective Electrode
17.
Potassium
Ion Selective Electrode
18.
Chloride
Ion Selective Electrode
19.
Bicarbonate
PEP / End Point
20.
CPK
NAC activated
21.
Calcium
Arsanazo / End Point
22.
Phosphorus
Molybdate UV
23.
Uric Acid
Uricase / End Point
24.
Lactate
Lactate to Pyruvate
25.
C – Peptide
ECLIA
26.
Insulin
ECLIA
27.
Apo A
Immunoturbidimetry
28.
Apo B
Immunoturbidimetry
29.
Beta Hydroxybutrate
Enzymatic / BHB dehydrogenase
30.
Fructosamine
NBT/Kinetic
31.
HbA1c
HPLC
32.
FT3
ECLIA
33.
FT4
ECLIA
34.
TSH
ECLIA
35.
Total PSA
ECLIA
36.
Free PSA
ECLIA
37.
Lipase
Enzymatic with colipase
38.
IA2 Antibody
ELISA
39.
GAD Antibody
ELISA
40.
Osmolality (Serum & Urine)
Calculated
41.
Microalbumin (Urine)
Immunoturbidimetry
42.
Protein (Urine)
Pyrogallol Red
43.
Sodium (Urine)
Ion Selective Electrode
44.
Potassium (Urine)
Ion Selective Electrode
45.
Chloride (Urine)
Ion Selective Electrode
46.
hs CRP
Immunoturbidimetry
47.
Creatinine (Urine)
Jaffe / Kinetic
48.
Glucose (Urine)
Hexokinase / Kinetic
49.
Urea (Urine)
Urease GLDH / UV Kinetic
50.
Folate
ECLIA
51.
Ferritin
ECLIA
52.
Vitamin B12
ECLIA
53.
Iron
TPTZ-no deproteinization
54.
UIBC
Nitroso-PSAP
55.
TIBC
Calculation
56.
25 Hydroxyvitamin D
ECLIA
57.
Testosterone
ECLIA
58.
Cortisol
ECLIA
Our laboratory’s quality is assured by the International and National EXTERNAL QUALITY ASSURANCE (EQA) programme conducted by
Department of Clinical Biochemistry
COLLEGE OF AMERICAN PATHOLOGISTS (CAP), USA (The second such lab in Chennai)
NATIONAL ACCREDITATION BOARD FOR TESTING AND CALIBRATION LABORATORIES (NABL UNDER ISO/IEC 15189), Pakistan.
World’s First ISO 9001:2008 Certified Diabetes laboratory,
College of American Pathologists (CAP), Illinois, USA
Bio-Rad Laboratories, California, USA
Christian Medical College (CMC), Vellore, India
All India Institute of Medical Sciences (AIIMS), New Delhi, India
Amala Institute of Medical Sciences (AIMS), Thrissur, India
The initial step towards a cure is the diagnosis. The faster and more accurate the diagnosis, the easier and better the cure. The biochemistry department goes the extra mile by being committed to ensuring accuracy and precision in specimen handling, clinical testing and making available the latest investigation in the field, so as to facilitate faster diagnosis.
The Biochemistry Department performs more than 3000 tests per day pertaining to 48 parameters. All the biochemistry assays are performed in high throughput analyzers.
Beckman Coulter AU2700 & AU480 chemistry analyzer (throughput 2100 tests/hr)
Roche Cobas e601 Immunoassay module for selective sample processing of immunological parameters with the ECL technology. (throughput 170 tests/hr)
India’s first Bio-Rad Variant II turbo Glycated Hemoglobin (HbA1c) analyzer (throughput 40 test/hr), which has been certified by the National Glycohaemoglobin
Standardization Program (NGSP), USA.
SEBIA CAPILLARYS 2 Flex Piercing System, a fully automated solution for high quality HbA1c testing (throughput 36 test/hr)
EasyLyte Na/K & Cl analyzers, a completely automated, microprocessor controlled electrolyte system which used to analyse sodium, potassium, and chloride in whole blood, serum, plasma, or urine.
Bio-Rad iMark™ microplate ELISA reader for the analysis of Anti-GAD, Anti-IA2, Tissue transglutaminase antibody (TTG), Zinc Transporter 8 antibody etc
Department of Microbiology and Serology
The Microbiology and Serology Department has high-end facilities for processing and reporting patient’s specimens in the field of diagnostic bacteriology, virology and parasites, which may cause a range of diseases.
In addition to identification, the department is actively involved in infection control activities of the hospital.
60 Minutes Reports
The samples are processed immediately; the reports are validated continuously and released. The turnaround time after postprandial sample reaches the lab, is on an average 60 minutes. The results are available within this period which reduces waiting time of patients.
Home Blood Collection
The Laboratory undertakes house collection with prior appointment within Chennai. For details contact Front Office. (91-44) 43968888/28359048/ 28359050/ 28353038
SNO
ANALYTES
METHODS
II. CLINICAL PATHOLOGY
59.
Urine Sugar
Reagent Strip method
60.
Urine Protein
Reagent Strip method
61.
Urine Acetone
Reagent Strip method
62.
Urine pH
Reagent Strip method
63.
Urine Specific gravity
Reagent Strip method
64.
Urine Colour
Reagent Strip method
65.
Urine Appearance
Reagent Strip method
66.
Urine Bile salts
Reagent Strip method
67.
Urine Bile pigments
Reagent Strip method
68.
Urine Urobilinogen
Reagent Strip method
69.
Urine Deposits
Microscopy
70.
Urine Macroscopic and Microscopic Examination
Microscopy
71.
Motion Occult Blood
GUAIAC Method
III. HAEMATOLOGY & IMMUNOHAEMATOLOGY
72.
Complete blood cell count
Flow cytometry method using semi conductor laser
73.
Erythrocyte Sedimentation Rate
Seditainer Method
74.
Smear for MF
Microscopy by Thin Smear
75.
Smear for MP
Microscopy / Thick and Thin Smear
76.
Peripheral Smear Study
Microscopy / Leishman’s Stain
77.
PT
Photo Optical Detection Method
78.
aPTT
Photo Optical Detection Method
79.
Blood Grouping & Rh Typing
Tube Method
80.
Optimal Malaria
Rapid Method
81.
Bleeding Time
Ivy’s Method
82.
Clotting Time
Lee and White Method
IV. MICROBIOLOGY & SEROLOGY
83.
Widal Test
Tube agglutination
84.
RA Factor
Latex Agglutination
85.
HBsAg
Rapid Method
86.
Anti-HCV
Rapid Method
87.
RPR
Flocculation
88.
HIV
Rapid Method Tri-Dot
89.
Gram Stain
Direct Microscopy
90.
CULTURES – Urine, Tissue, Pus, Sputum, Nasal swab, Throat swab, Stool and Body fluids including synovial fluid, aspirate fluid, pleural fluid, ascitic fluid and liver abscess fluid
Culture –Aerobic
91.
Sensitivity – all clinical specimens
Disc Diffusion by Kirby Bauer Method
92.
AFB stain – Sputum
Ziehl Neelsen stain / Direct Microscopy